Transcriptional Regulation: Effects of Promoter Proximal Pausing on Speed, Synchrony and Reliability
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چکیده
منابع مشابه
Transcriptional Regulation: Effects of Promoter Proximal Pausing on Speed, Synchrony and Reliability
Recent whole genome polymerase binding assays in the Drosophila embryo have shown that a substantial proportion of uninduced genes have pre-assembled RNA polymerase-II transcription initiation complex (PIC) bound to their promoters. These constitute a subset of promoter proximally paused genes for which mRNA elongation instead of promoter access is regulated. This difference can be described as...
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The transcriptional regulation of a eukaryotic gene is specified by the interplay of specific regulatory factors, general transcription factors (GTFs), RNA polymerase II (Pol II), DNA sequence elements, and the chromatin structure of the promoter. The thousands of genes in a eukaryotic cell share a general transcriptional machinery and a common transcriptional pathway (Orphanides et al. 1996). ...
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The RNA Pol II transcription complex pauses just downstream of the promoter in a significant fraction of human genes. The local features of genomic structure that contribute to pausing have not been defined. Here, we show that genes that pause are more G-rich within the region flanking the transcription start site (TSS) than RefSeq genes or non-paused genes. We show that enrichment of binding m...
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MOTIVATION It has been known for more than 2 decades that after RNA polymerase II (RNAPII) initiates transcription, it can enter into a paused or stalled state immediately downstream of the transcription start site before productive elongation. Recent advances in high-throughput genomic technologies facilitated the discovery that RNAPII pausing at promoters is a widespread physiologically regul...
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ژورنال
عنوان ژورنال: PLoS Computational Biology
سال: 2011
ISSN: 1553-7358
DOI: 10.1371/journal.pcbi.1001136